Which pigment is the most nonpolar

Lower Rf values mean the pigment is more polar. Draw or tape your TLC strip and label as many pigments as you can see the next page for more information on pigments. Record the Rf values of each pigment next to its label. Which pigment is more polar, chlorophyll a or chlorophyll b? How can you tell? Which pigments were the most nonpolar least polar, highest Rf values?

If there were polar pigments in the leaves and you used a nonpolar solvent to extract the pigments from the leaf, would they dissolve and be present in the solution you used to run your TLC experiment? How might this impact your results? Maria Morrow College of the Redwoods. Materials needed: Mortar and pestle Sand or similar material Soft leaves e.

Procedure for Thin Layer Chromatography Extracting the pigments. Prepare your TLC strip by drawing a line across the paper in pencil 2 cm from the bottom of the strip and set aside.

Important note : Handle the strip as little as possible so oils from your hands do not interfere with the process. Under a hood or in a well-ventilated room, put some of the leaves into the mortar with a little bit of sand to help break the tissue apart and some extraction solvent. Grind the leaves with the pestle until they have turned to mush. You may need to add more extraction solvent as it soaks into the leaf tissue.

A pigment that is the most soluble will travel the greatest distance and a pigment that is less soluble will move a shorter distance.

If you were to perform your experiment on a chromatography strip that was twice the length of the one you used, would your Rf values still be the same?

The Rf value represents the ratio of the distance a pigment moved on the chromatogram relative to the distance the solvent front moved. As the solvent crosses the area containing plant pigment extract, the pigments dissolve in and move with the solvent. The pigments are carried along at different rates because they are not equally soluble.

Therefore, the less soluble pigments will move slower up the paper than the more soluble pigments. Leaves contain different pigments, which give them their color. Green chlorophyll is the most common type of pigment, but there are also carotenoids yellow, orange and anthocyanins red. Leaves are green because of the green pigment called chlorophyll which is involved in photosynthesis. Chlorophyll a, which is the major pigment in green , leafy plants, gives spinach its green color.

Which one is the most non polar spinach pigment? Category: healthy living nutrition. Carotene moves the farthest because it is the most nonpolar of the pigments and it is attracted more strongly to the acetone-ligroin mixture mobile phase than to the paper.

This stronger, nonbonded interaction with the mobile phase indicates that carotene is the most nonpolar pigment found in spinach chloroplasts. What is the most polar pigment? How many pigments are in a leaf? Can an RF be greater than 1? Is water polar or nonpolar? Why is an RF value a dimensionless number? Which is more polar Xanthophyll or beta carotene? At the top of our chlorophyll -containing WHFoods you will find spinach. This vegetable contains about 24 milligrams of chlorophyll per cup raw.

How do you calculate RF? Calculating Retention Factor. Lokapala Mordkovich Supporter. Why do most leaves look green? Green plants are green because they contain a pigment called chlorophyll. As shown in detail in the absorption spectra, chlorophyll absorbs light in the red long wavelength and the blue short wavelength regions of the visible light spectrum.

Green light is not absorbed but reflected, making the plant appear green. Simon Thiesing Beginner. What does higher RF value mean? A high Rf Ie 0. A low Rf value 0.

Spaska Telaar Beginner. Does a higher Rf value mean more polar? In general, the adsorptivity of compounds increases with increased polarity i. Non- polar compounds move up the plate most rapidly higher Rf value , whereas polar substances travel up the TLC plate slowly or not at all lower Rf value. Magomed Khalifa Beginner. How many pigments are in a spinach leaf? Then there are carotenoids which include pigments anywhere from red to orange-yellow.

Xanthophylls- oxidized versions of carotenes, play a secondary role in photosynthesis. Adilia Wernicke Beginner. Does RF value change with different solvents? As, mentioned by Jan Increasing the polarity of solvent will result in increase in Rf value as the compound in even for polar or nonpolar compound, this is the rule of thumb in most cases, may be except do exist i don't know about it.

But for my experience in the laboratory polarity result increase in Rf value. Ask A Question. Co-authors: 3.